Partial Peptide of α-Synuclein Modified with Small-Molecule Inhibitors Specifically Inhibits Amyloid Fibrillation of α-Synuclein
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of Proteolytic-Digested α-Synuclein Partial Peptide Sequence
2.2. Evaluation of Inhibitory Effects of PQQ-Modified Peptides on Amyloid Formation of α-Synuclein
2.3. Evaluation of Specificity of PQQ-Modified α-Syn36–46 Peptide
2.4. Evaluation of Inhibitory Effects of Baicalein or EGCG-Modified α-Syn36–46 Peptide on Amyloid Formation of α-Synuclein
3. Experimental Section
3.1. Preparation of Recombinant α-Synuclein
3.2. LC/MS Analysis of Proteolytic-Digested α-Synuclein
3.3. PQQ, Baicalein, EGCG-Modification of α-Syn Partial Peptides
3.4. Amyloid Fibril Formation Analysis
3.5. Cytotoxicity Assay of α-Synuclein
4. Conclusions
Acknowledgments
References
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Position | Sequence |
---|---|
3–13 | VFMKGLSKAKE |
21–28 | KTKQGVAE |
36–46 | GVLYVGSKTKE |
47–57 | GVVHGVATVAE |
127–131 | MPSEE |
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Yoshida, W.; Kobayashi, N.; Sasaki, Y.; Ikebukuro, K.; Sode, K. Partial Peptide of α-Synuclein Modified with Small-Molecule Inhibitors Specifically Inhibits Amyloid Fibrillation of α-Synuclein. Int. J. Mol. Sci. 2013, 14, 2590-2600. https://doi.org/10.3390/ijms14022590
Yoshida W, Kobayashi N, Sasaki Y, Ikebukuro K, Sode K. Partial Peptide of α-Synuclein Modified with Small-Molecule Inhibitors Specifically Inhibits Amyloid Fibrillation of α-Synuclein. International Journal of Molecular Sciences. 2013; 14(2):2590-2600. https://doi.org/10.3390/ijms14022590
Chicago/Turabian StyleYoshida, Wataru, Natsuki Kobayashi, Yasuhiko Sasaki, Kazunori Ikebukuro, and Koji Sode. 2013. "Partial Peptide of α-Synuclein Modified with Small-Molecule Inhibitors Specifically Inhibits Amyloid Fibrillation of α-Synuclein" International Journal of Molecular Sciences 14, no. 2: 2590-2600. https://doi.org/10.3390/ijms14022590